Yogesh S. Shouche, Ph. D.

 

 

 

 

 

 

Research Area & Interests :  

Environmental and Evolutionary genomics.

My laboratory is involved in understanding the microbial community structure of some unique environmental sites using the 16S rRNA based culture independent approach. We have studied the mid gut flora of mosquito Culex quinquefasciatus and described a new species Aeromonas culicicola from it. Presently we are studying the role played by this species in the gut environment. We have undertaken major efforts to analyze Expressed Sequence Tags in the mid gut of malaria vector Anopheles stephensii.  

In addition we are also analyzing marine ecosystems and gut environments of other insects to understand their diversity. We are using various molecular chronometers (16S rRNA, 23S rRNA, gyrB, rpoD) to study evolutionary relationships between diverse groups of organisms, like bacteria, fish, reptiles and insects.

Current Projects :

  1. Microbial community structure and dynamics in unique ecosystems.

  2. Genetic Regulatory circuits and virulence factors in Aeromonas culicicola.

  3. Gene regulation in mosquitoes in relation to development and host-parasite interaction.

Projects with extra mural funding:

  1. Genetic diversity of Helicobacter pylori isolates from patients of Indian origin, funded by Department of Biotechnology.

  2. Studies on Genetic Relatedness of Bacillus anthracis Strains of Indian Origin, funded by Life Sciences Research Board.

  3. Expressed Sequence Tag analysis of mosquito genome, funded by Department of Biotechnology.

  4. Looking for evidence for life in outer space: Studies on meteor crators, funded by Indian Space Research Organization.

  5. Investigation of the microflora of insets of Western Ghats for potentially useful bioactive molecules, funded by Department of Biotechnology.

  6. Molecular characterization of microbial and invertebrate diversity of Indian West Coast, funded by Department of Biotechnology.

 

Paper Published :

Journal and Review articles: 33      

Abstracts: 19

Recent  Papers:

  1. Pidiyar, V.J, Jangid, K., Patole, M.S., and Shouche, Y.S. 2004. Studies on cultured and uncultured microbiota of wild Culex quinquefasciatus mosquito midgut based on 16S rRNA gene analysis. American Journal of Tropical Medicine and Hygiene. 70: 597-603. 

  2. Dighe, A.S., Jangid, K., Gonzalez, J.M., Pidiyar, V.J., Patole, M.S., Ranade, D.R., and Shouche, Y.S. 2004 Comparison of 16S rRNA gene sequences of genus Methanobrevibacter. BMC Microbiology. 4: 20.

  3. Jangid, K., Rastogi, G., Patole, M.S., and Shouche, Y.S. 2004. Methanobrevibacter: Is it a potential pathogen. Current Science. 86: 1475-1476.

  4. Jaykumar, P.C., Musande, V., Shouche, Y.S., and Patole, M.S. 2004. The Selenophosphatase gene from Leishmania major. DNA Sequence. 15: 66-70.

  5. Pidiyar, V.J., Jangid, K., Patole, M.S., and Shouche, Y.S. 2003. Analysis of 16S-23S intergenic spacer regions and rrn operon copy number of Aeromonas culicicola MTCC 3249T. DNA Sequence. 14: 183-194.

  6. Pidiyar, V.J., Jangid, K., Dayanand, K.M., Patole, M.S., Gonzales, J.M., Kaznowski, A., and Shouche, Y.S. 2003. Phylogenetic affiliation of Aeromonas culicicola MTCC 3249T based on gyrB gene sequence and PCR–Amplicon Sequence Analysis of cytolytic enterotoxin gene. Systematic and Applied Microbiology. 26: 197-202.

  7. Pidiyar, V.J., Jangid, K., Patole, M.S., and Shouche, Y.S. 2003. Detection and phylogenetic affiliation of Wolbachia sp. from Indian mosquitoes Culex quinquefasciatus and Aedes albopictus. Current Science 84: 1136-1139

  8. Mourya, D.T., Singh, D.K., Yadav, P., Gokhale, M.D., Barde, P.V., Jayakumar, P.C., Thakare, J.P., Mishra, A.C., and Shouche, Y.S. 2003. Role of gregarine parasite Ascogregarina culicis (Apicomplexa: Lecudinidae) in the maintenance and vertical transmission of Chikungunya virus in the vector mosquito Aedes aegypti. Journal of Eukaryotic Microbiology 50: 379-382.

  9. Yadav, P., Shouche, Y.S., Munot, H.P., Mishra, A.C., and Mourya, D.T. 2003. Genotyping of Chikungunya virus isolated in India during 1963-2000; by reverse transcription polymerase chain reaction. Acta Virologica. 47: 125-127.

  10. Pidiyar, V., Kaznowski, A., Narayan, N.B., Patole, M.S., and Shouche, Y.S. 2002. Aeromonas culicicola sp. nov. (MTCC 3249T), from the midgut of Culex quinquefasciatus. International Journal of Systematic and Evolutionary Microbiology. 52: 1723-1728.

  11. Savant, D.V., Shouche, Y. S., Prakash, S., and Ranade, D.R. 2002. Methanobrevibacter acidophilicus sp. nov.  a new methanogen from sour anaerobic digester. International Journal of Systematic and Evolutionary Microbiology. 52: 1081-1087.

  12. Mourya DT, Gokhale MD, Pidiyar V, Barde PV, Patole M, Mishra AC, Shouche Y. 2002. Study of the effect of the midgut bacterial flora of Culex quinquefasciatus on the susceptibility of mosquitoes to Japanese encephalitis virus. Acta Virologica. 46: 257-260.

Recent ABSTRACTS / Papers Presented:

  1. Jangid, K., Dighe, A.S., Gonzalez, J.M., Pidiyar, V., Patole, M.S., and Shouche Y.S. 2004. Comparison of 16S rRNA gene sequences of genus Methanobrevibacter. Extremophiles 2004: 5th International Conference on Extremophiles (An ASM conference). Cambridge, Maryland, USA.

  2. Jangid, K., Pidiyar, V., Gonzalez, J.M., Kaznowski, A., Patole, M.S., and Shouche Y.S. 2004. Gene sequence analysis and AFLP: An answer to the confusing Aeromonas taxonomy. 8th ADNAT Convention and Symposium on Functional Genomics. Hyderabad, India.

  3. Rastogi, G., Ranade, D.R., Patole, M.S., and Shouche, Y.S. 2003.  Microbial Population Genomics and Ecology of Anaerobic Digestion Ecosystem. International Symposium on Molecular Taxonomy and Environmental Health. Lucknow, India.

  4. Kerkar, S., Shouche, Y.S., Nair, S., and Bharati, L. 2003. Hypersaline Sulfate-Reducing bacteria from salt pans: Mixed response to mercury. Marine Biotechnology Conference. Chiba, Japan.

  5. Pidiyar, V., Kaznowski, A., Narayan, N.B., Jangid, K., Dayanand, K. M., Musande, V., Patole, M.S., and Shouche, Y.S. 2002. Isolation of Aeromonas culicicola sp. nov. (MTCC 3249T), from the midgut of Culex quinquefasciatus and its interaction with the host. 7th International Symposium of Aermomonas and Plesiomonas. Orihuela, Spain.

Student Information :

Presently Working:

  1. Dr. Rajni Kant Dixit                 (RA, dixit2k@yahoo.com)

  2. Dr. Upal Roy                           (RA, upal123@rediffmail.com)

  3. Mr. N. Badri Narayan              (SRF, badri@nccs.res.in)

  4. Mr. Gurdeep Rastogi             (SRF, gurdeeprastogi@nccs.res.in)

  5. Mr. K.M. Dayanand                 (SRF, dayanand@nccs.res.in)

  6. Mr. Kamlesh Jangid               (SRF, jangidk@nccs.res.in)

  7. Mr. Mahesh Dharne                (JRF, star_mahesh@yahoo.co.in)

  8. Mr. Kaushal Shrama               (JRF, send2kaushal@yahoo.co.in)

  9. Mr. Aijaz Ahmed Wani            (JRF, aijaz_ah@rediffmail.com)

  10. Mr. Jay Siddharth                    (JRF, jaysiddharth@rediffmail.com)

  11. Mr. Kaustubh Gokhale            (JRF, kaustubh@nccs.res.in)

  12. Mr. D. Ananthram                (JRF, nirvikalpa@rediffmail.com)

  13. Mr. Rasesh Parikh               (JRF, reseshparikh@yahoo.com)

  14. Mr. Pankaj Verma            (JRF, pankajbiotech2002@rediffmail.com)

  15. Mr. Ashraf Rangrez                    (JRF, ashraf_ran@rediffmail.com)

  16. Dr. Satinder Singh            (JRF, sonuvets@hotmail.com)

  17. Mr. Rajesh Kundapur                  (Project Assistant, getraj_shiv@yahoo.co.in)

  18. Mr. Pradip Ghare                  (Project Assistant, pradip_gar@yahoo.com)

  19. Mr. Sarang Satoor                   (Technician, snsatoor@nccs.res.in)

Past Students:

  1. Dr. Abhijit S Dighe                   (PDF, Currently at University of Virginia, USA)

  2. Dr. Vyankatesh J Pidiyar         (PDF, Currently at University of Virginia, USA)

Contact Info : 

National Centre for Cell Science

NCCS Complex, GaneshKhind

Pune - 411 007, Maharashtra

Ph.: 020 - 25690922, 25690941 / 51 / 61 extn.: 210 (Lab.), 273 (Res.)

        020 - 25697764 (Res.)

Fax: 020 - 25692259

E-mail : yogesh@nccs.res.in 

Miscellaneous Information / Other Activities :

Invited Talks:

At various national and international scientific meetings and conferences on topics related to Genomics, Microbial Diversity, Molecular Phylogenetics, Environmental Biotechnology

Popular Talks:

On topics like Bioterrorism; Genes, Genomes and Genomics; Human Genome; and DNA Fingerprinting at various universities and colleges all over India.

Radio talks: 

On AIR, Pune station on topics like Extraterrestrial Life and Premature ageing.