Manoj Kumar Bhat, Ph.D.
Ph.D. Biochemistry, 1992, University of Mysore, India
M.Sc. Biochemistry, 1985, University of Mysore, India
B.Sc. Chemistry, Physics and Mathematics, 1983, University of Mysore, India
Scientist D to G, 1998 to present, National Centre for Cell Science, Pune, India
Research Associate, 1998-1998, Institute of Human Virology, University of Maryland, Maryland
Visiting Fellow, 1993-1998, National Cancer Institute, NIH, Bethesda, MD
Senior Research Fellow, 1990-1992, University of Mysore, India
Junior Research Fellow, 1986-1989, University of Mysore, India
Cell Biology and Cancer Biology/Chemotherapy
Khalique, A, Sarwade, R.D., Pandey, P.R., Vijayakumar, M.V., Bhat, M.K. and Seshadri, V.: Prolonged exposure to insulin with insufficient glucose leads to impaired Glut4 translocation. Biochemical and Biophysical Research Communications, 474(1):64-70, (2016).
Chouhan, S., Singh, S., Ramteke, P., Athavale, D., Pandey, V., Joseph,J., Mohan, R., Shetty, P.K. and Bhat, M.K.: Glucose induced activation of canonical Wnt signaling pathway in hepatocellular carcinoma is regulated by DKK4. Scientific Reports ( Accepted May), (2016).
Chaube, B. Bhat, M.K.: AMPK, a key regulator of metabolic/energy homeostasis and mitochondrial biogenesis in cancer cells. Cell Death and Disease, 7:e2044, (2016).
Chaube, B., Malvi, P., Singh, S.V., Mohammad, Viollet, B. and Bhat, M.K.: AMPK maintains energy homeostasis and survival in cancer cells under metabolic stress by regulating p38/PGC-1α-mediated mitochondrial biogenesis. Cell Death and Discovery, 1, 15063, (2015).
Chaube, B., Malvi, P., Singh, S.V., Mohammad, N., Meena, A.S. and Bhat, M.K.: Targeting metabolic flexibility by simultaneously inhibiting respiratory complex I and lactate generation retards melanoma progression. Oncotarget, 6(35):37281-37299, (2015).
Singh, S.V., Ajay, A.K., Mohammad, N., Malvi, P., Chaube, B., Meena, A.V. and Bhat, M.K.: Proteasomal inhibition sensitizes cervical cancer cells to mitomycin C induced bystander effect: The role of tumor microenvironment. Cell Death and Disease, 6:e1934, (2015).
Mohammad, N., Singh, S.V., Malvi, P., Chaube, B., Athavale, D.A., Vanuopadath, M., Nair, S.S., Nair, B.G. and Bhat, M.K.: Strategy to enhance the efficacy of doxorubicin in breast and hepatocellular carcinoma by Methyl-β-cyclodextrin: Molecular events involved. Scientific Reports, 5, 11853, (2015).
Malvi, P., Chaube, B., Pandey, V., Vijayakumar, M.V., Boreddy, P.V., Mohammad, N., Singh, S.V. and Bhat, M.K.: Obesity induced rapid melanoma progression is reversed by orlistat treatment and dietary intervention: role of adipokines. Molecular Oncology, 9(3):689-703, (2015).
Malvi, P., Piprode, V., Chaube, B., Pote, S.T., Mittal, M., Chattopadhyay, N., Wani, M.R. and Bhat, M.K.: High fat diet promotes achievement of peak bone mass in young rats. Biochemical and Biophysical Research Communications, 455(1-2):133-8, (2014).
Mohammad, N., Malvi, P., Meena, A.S., Singh, S.V., Chaube, B., Vannuruswamy, G., Kulkarni, M.J and Bhat M.K.: Cholesterol depletion by methyl-β-cyclodextrin augments tamoxifen induced cell death by enhancing its uptake in melanoma. Molecular Cancer, 13:204, (2014).
Kumar, R., Sinha, A., Vijayakumar, M.V., Pandey, V., Bhide A., Javadekar, S.S., Pawar, B. and Bhat, M.K.: Population based estimation of mortality and occurrence of cancer in patients with and without diabetes in Pune City. Current Science, 105(4): 486-491 and, In this issue section page 426, (2013).
Meena, AS., Sharma, A., Kumari, R., Mohammad, N., Singh, S.V. and Bhat, M.K.: Inherent and acquired resistance to paclitaxel in hepatocellular carcinoma: Molecular events involved. PLOS One, 8(4):e61524, (2013).
Kumar, A., Mitra, A., Ajay, A.K., Bhat, M.K. and Rao, C.P.: Cu(II) complexes of glyco-imino-aromatic conjugates in DNA binding, plasmid cleavage and cell cytotoxicity.Journal of Chemical Sciences, 124(6): 1217-1228, (2013).
Parvatkar, P.T., Ajay, A.K., Bhat, M.K., Parameswaran, P.S., Tilve, S.G.: Iodine catalyzed one-pot synthesis of chloro-substituted linear and angular indoloquinolines and in vitro antiproliferative activity study of different indoloquinolines. Medicinal Chemistry Research, 22:88–93, (2013).
Pandey V., Vijayakumar, M.V., Ajay, A.K. Malvi, P. and Bhat, M.K.: Diet induced obesity increases melanoma progression: involvement of Cav-1 and FASN. International Journal of Cancer, 130(3):497-508, (2012).
Ajay, A.K., Meena A.S. and Bhat, M.K.: Human papillomavirus 18 E6 inhibits phosphorylation of overexpressed p53 in HeLa cells. Cell and Bioscience, 2:2, (2012).
Vijayakumar, M.V. and Bhat, M. K.: Real time qualitative and quantitative Glut4 translocation assay. Methods in Enzymology “Imaging and Spectroscopy of Live Cells”, 505:257-71,(2012)
Sharma, A. and Bhat M.K.: Enhancement of Carboplatin and Quercetin Induced Cell Death by Roscovitine is Akt Dependent and p53 Independent in Hepatoma Cells. Integrated Cancer Therapies, 10(4):NP4-14, (2011).
Pandey V., Chaube, B. and Bhat, M.K.: Hyperglycemia regulates MDR-1, drug uptake and ROS levels causing increased toxicity of carboplatin and 5-fluorouracil in MCF-7 cells. Journal of Cellular Biochemistry, 112(10):2942-52, (2011).
Kumar, A., Chinta, J.P., Ajay, A.K., Bhat, M.K. and Rao, C.P.: Synthesis, characterization, plasmid cleavage and cytotoxicity of cancer cells by a copper(II) complex of anthracenyl–terpyridine. Dalton Transactions, 40(41):10865-72, (2011).
Ajay, A.K, Upadhyay, A.K., Singh S., Vijayakumar, M.V., Kumari, R., Pandey, V., Boppana, R. and Bhat M.K.: Cdk5 Phosphorylates Non-genotoxically Overexpressed p53 following Inhibition of PP2A to Induce Cell Cycle Arrest/apoptosis and Inhibits Tumor Progression. Molecular Cancer, 9:204, (2011).
Vijayakumar, M.V., Ajay, A.K. and and Bhat, M.K.: Demonstration of a visual cell-based assay for screening glucose transporter 4 translocation modulators in real time.Journal of Biosciences, 35(4):525-31, (2010).
Sharma, A. Meena A.S. and Bhat, M.K.: Hyperthermia associated carboplatin resistance: Differential role of p53, HSF1 and Hsp70 in hepatoma Cells. Cancer Science, 101(5):1186-93, (2010).
Shriram, V., Kumar, V., Suryawanshi, S., Upadhyay, A. and Bhat, M.K.: Cytotoxic activity of 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda against human cancer cells. Journal of Ethnopharmacology, 128(1):251-3, (2010).
Vijayakumar, M.V., Pande, V, Mishra, G.C. and Bhat, M.K.: Hypolipidemic effect of fenugreek seeds is mediated through inhibition of fat accumulation and up regulation of LDL receptor. Obesity, 18(4):667-74, (2010).
Kumari, R. Sharma, A., Ajay, A.K. and Bhat, M.K.: Mitomycin C induced bystander killing in homogenous and heterogeneous cell model of hepatoma cells: mechanism and significance. Molecular Cancer, 8:87, (2009).
Sharma, A., Upadhyay, A.K. and Bhat, M.K.: Inhibition of Hsp27 and Hsp40 potentiates 5-fluorouracil and carboplatin mediated cell killing in hepatoma cells. Cancer Biology and Therapy, 8(22):2106-2113, (2009).
Aher, N.G., Pore, V.S., Mishra, N.N., Kumar, A., Shukla, P.K., Sharma, A., and Bhat, M.K.: Synthesis and antifungal activity of 1,2,3-triazole containing new fluconazole analogues. Bioorganic Medicinal Chemistry Letters, 19: 759–763, (2009).
Pandey, V., Vijayakumar, M.V., Kaul-Ghanekar, R., Mamgain, H., Paknikar, K. and Bhat, M.K.: Atomic Force Microscopy, biochemical analysis of 3T3-L1 cells differentiated in the absence and presence of insulin. Biochimica et Biophysica Acta, 1790(1):57–64, (2009).
Upadhyay, A.K., Ajay, A.K., Singh, S. and Bhat, M.K.: Cell Cycle Regulatory Protein 5 (Cdk5) is a Novel Downstream Target of ERK in Carboplatin Induced Death of Breast Cancer Cells. Current Cancer Drug Targets, 8(8):741-752, (2008).
Vijayakumar, M.V. and Bhat, M.K.: Hypoglycemic effect of dialyzed fenugreek seeds extract is sustainable and is mediated, in part, by the activation of hepatic enzymes. Phytotherapy Research, 22: 500-525, (2008).
Gholap, A. R., Toti, K. S., Shirazi, F., Kumari R., Bhat, M.K., Deshpande, M.V. and Srinivasan K.V.: Synthesis and evaluation of antifungal properties of a series of the novel 2-amino-5-oxo-4-phenyl-5,6,7,8-tetrahydroquinoline-3-carbonitrile and its analogues. Bioorganic and Medicinal Chemistry, 15(21): 6705-6715, (2007).
Chhipa, R.R., Kumari, R.Upadhyay, A.K. and Bhat, M.K.: Abrogation of p53 by its antisense in MCF-7 breast carcinoma cells increases cyclin D1 via activation of Akt and promoting cell proliferation. Experimental Cell Research, 15; 313(19): 3945-3958, (2007).
Singh, S., Upadhyay, A.K., Ajay, A.K. and Bhat, M.K.: p53 regulates ERK activation in carboplatin induced apoptosis in cervical carcinoma: A novel target of p53 in apoptosis. FEBS Letters, 581: 289–295, (2007).
Chhipa, R.R. and Bhat, M.K.: Bystander killing of breast cancer MCF-7 cells by MDA-MB-231 cells exposed to 5-fluorouracil is mediated via Fas. Journal of Cellular Biochemistry, 101: 68–79, (2007).
Singh, S., Upadhyay, A.K., Ajay, A.K. and Bhat, M.K.: Gadd45a does not modulate the cytotoxicity of DNA damaging drugs in HPV-positive cells even though it is induced in response to drug treatment. Journal of Cellular Biochemistry, 100: 1191–1199, (2007).
Upadhyay, A.K., Singh, S., Ajay, A.K. and Bhat, M.K.: Methyl-β-cyclodextrin enhances the susceptibility of human breast cancer cells to carboplatin and 5-fluorouracil: Involvement of Akt, NF-kappaB and Bcl-2. Toxicology and Applied Pharmacology, 216(2):177-185, (2006).
Singh, S., Chhipa, R.R., Vijayakumar M.V. and Bhat, M.K.: DNA damaging drugs induced downregulation of Bcl-2 is essential for induction of apoptosis in high-risk HPV-positive HEp-2 and KB cells. Cancer Letters, 236 (2): 213-221, (2006).
Vijayakumar M.V., Singh, S., Chhipa, R.R. and Bhat, M.K.: Hypoglycemic activity of fenugreek seeds extract is mediated through the stimulation of insulin signaling pathway. British Journal of Pharmacology, 146 (1): 41-48, (2005).
Chhipa, R.R., Singh, S., Surve, S.V., Vijayakumar M.V. and Bhat, M.K.: Doxycycline potentiates antitumor effect of cyclophosphamide in mice. Toxicology and Applied Pharmacology, 202(3):268-277, (2005).
Singh, S. and Bhat, M.K.: Carboplatin induces apoptotic cell death through downregulation of constitutive activity of nuclear factor-kB in human HPV-18 E6 positive HEp-2 cells. Biochemical and Biophysical Research Communications, 318: 346-353, (2004).
Salunke, D.B., Hazra B.J., Pore V.S., Bhat, M.K., Nahar, P.B. and Deshpande, M.V.: New Steroidal Dimers with Antifungal and Antiproliferative Activity. Journal of Medicinal Chemistry, 47: 1591-1594, (2004).
Kaul, R., Ahmed, F., Bhat M.K., Chhipa, R., Galande, S. and Chattopadhyay, S.: Direct interaction and activation of p53 by SMAR1 causes cell cycle arrest at G2M phase and delays tumor growth in mice. International Journal of Cancer, 103: 606-615, (2002).
Zhu, X-Y., Kaneshige, M., Bhat, M.K., Zhu, Q., Mariash, C.N., McPhie, M. and Cheng, S-Y.: The orphan nuclear receptor Ear-2 is a negative Co-regulator for thyroid hormone nuclear receptor function. Molecular and Cellular Biology, 20(7): 2604-2618, (2000).
Bhat, M.K., Dace A. and Cheng, S-Y.: Tissue -specific differential repression of gene expression by a dominant negative mutant of thyroid hormone β1 receptor. Thyroid, 9(4): 411-418, (1999).
Bhat, M.K., Yu, C-l., Yap, N., Zhan, Q., Hayashi, Y., Seth, P. and Cheng, S-y.: The tumor suppressor p53 is a negative regulator in thyroid hormone receptor signalling pathways. Journal of Biological Chemistry, 272: 28989-28996, (1997).
Bhat, M.K., Mcphie, P. and Cheng, S-Y.: The critical role of glutamine 252 in the hormone-dependent transcriptional activity of the thyroid hormone β1 nuclear receptor. Biochemistry, 36: 4233-4239, (1997).
Ting, Y.-T., Bhat, M.K., Wong, R. and Cheng, S.-Y.: Tissue-specific stabilization of the thyroid hormone β1 nuclear receptor by phosphorylation. Journal of Biological Chemistry, 272: 4129-4134, (1997).
Bhat, M.K., McPhie, P., Ting, T.-Y., Zhu, X.-G. and Cheng, S.-Y.: The structure of the carboxy-terminal region of thyroid hormone nuclear receptor and its possible role in hormone dependent intermolecular interactions. Biochemistry, 34: 10591-10599, (1995).
Bhat, M.K., McPhie, P. and Cheng, S.-Y.: Interaction of thyroid hormone nuclear receptor with antibody: Characterization of the thyroid hormone binding site. Biochemical and Biophysical Research Communications, 210: 464-471, (1995).
Bhat, M. K., Ashizawa, K. and Cheng, S.-Y.: Phosphorylation enhances the target gene sequence-dependent dimerization of thyroid hormone receptor with retinoid X receptor. Proceedings of National Academy of Science, USA. 91: 7927-7391, (1994).
Cheng, S.-Y., Ransom, S. C., McPhie, P., Bhat, M. K., Mixson, A. J. and Weintraub, B. D.: Analysis of the binding of 3,3',5-Triiodo-L-thyronine and its analogs to mutant human β1 thyroid hormone receptors: a model of the hormone binding site. Biochemistry, 33: 4319-4326, (1994).
Bhat, M. K., Parkison, C., McPhie, P., Liang, C. M. and Cheng, S.-Y.: Conformational changes of human β1 thyroid hormone receptor induced by binding of 3, 3’, 5-Triiodo-L-Thyronine. Biochemical and Biophysical Research Communications, 195: 385-392, (1993).
Bhat, M. K., and Gowda, T. V.: Indian cobra (Naja naja naja) venom phospholipases A2- Isolation, purification, and characterization. Mysore University Science Journal, XXXII: 467-474, (1992).
Bhat, M. K. and Gowda, T. V.: Purification of a lethal phospholipase A2 (NN-IVb1-PLA2) from the Indian cobra (Naja naja naja) venom. Biochemistry International, 25: 1023-1034, (1991).
Bhat, M. K., Kasturi, S., and Gowda, T. V.: Structure-function relationships among neurotoxic phospholipases: NN-X111-PLA2 from Indian cobra (Naja naja naja) and VRV-PL-V from Russell's viper (Vipera russelli) venoms. Toxicon, 29: 97-105, (1991).
Bhat, M. K., Prasad, B. N., and Gowda, T. V. Purification and characterization of a neurotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom. Toxicon, 29: 1345-1349, (1991).
Bhat, M. K., and Gowda, T. V.: Purification and characterization of myotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom. Toxicon, 27: 861-873, (1989).
An invited article titled Strategy to develop new drugs Diabetes and Identification of new targets among the signaling path in the development of Diabetes by Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat, has been published in books entitled: Chronic Inflammatory Diseases and Nutraceuticals (Molecular Mechanism of Chronic Diseases, New Targets & Drugs and Basic Concept of Ayurveda). Edited by Yamini B. Tripathi, Pages 257-266, (2005).
Method of preparing dialysed extract of fenugreek seeds which induces hypoglycemia, mediated, in part, via stimulation of insulin signaling pathway.
Bhat , M.K. and Vijayakumar, M.V.,US Patent issue No. 8865237- dated: 21-10-12014
Protein based product from fenugreek seeds that regulates dyslipidemia and obesity, and a process for the preparation thereof.
Bhat , M.K. Pandey, V. and Vijayakumar, M.V., European Patent issue No. EP2323676- dated: 15-07-2015 (Germany and France); (PCT/IN2008/000877 dated: 30-09-2008, WO/2009/157013, dated: 30-12-09).
Recipient of Government of India National Merit Scholarship, 1983
Junior Research Fellowship, Indian Council of Medical research and University Grants Commission, 1986-87
Senior Research Fellowship, University Grants Commission, India, 1990
Fogarty International Research Fellow, 1993-1998, NCI, NIH, USA
Fellow, The National Academy of Sciences, India, 2011
Nominated for DST supported Khorana Program Technology Transfer Course 2012 under IUSSTF (University of Wisconsin 14/15 July-28/29 July 2012)
Member, Molecular Immunology Forum (India)
Indian Association for Cancer Research
Indian Society of Cell Biology
Mr. Vijayakumar M.V.
Present Group - 2016
Left to right- Balkrishna, Shyamananda, Dipti, Abhijeet, Pranay, Surbhi, Ankita, Bhavana, Sneha